Certified Hyperbaric Technologist Practice Test

What is the Gram classification of C. perfringens?

1. Gram negative

2. Gram positive

3. Gram variable

4. Gram stain non-reactive

The correct answer is: Gram positive

C. perfringens is classified as Gram positive due to its thick peptidoglycan layer in the cell wall, which retains the crystal violet stain used during the Gram staining process. This thick layer is characteristic of Gram-positive bacteria, allowing them to appear purple under a microscope after staining. The presence of this outer layer contributes to the overall strength and structure of the bacterium, making it more resistant to certain environmental conditions. This classification is crucial for understanding the bacteria's behavior, including its pathogenicity and how it might react to different antibiotics. In contrast, Gram-negative bacteria have a thinner peptidoglycan layer and an outer membrane, which gives them different staining properties and resistance profiles.